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A simple method for removing stain precipitates from biological sections for transmission electron microscopy
Author(s) -
Kuo John
Publication year - 1980
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1980.tb04140.x
Subject(s) - transmission electron microscopy , stain , simple (philosophy) , biological specimen , electron microscope , microscopy , materials science , negative stain , chemistry , optics , nanotechnology , physics , staining , pathology , medicine , philosophy , epistemology
SUMMARY Stain precipitates originating from either lead salt or double staining with uranyl acetate and lead citrate on ultrathin sections of biological material can be removed by using 10% aqueous glacial acetic acid.