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An improved direct intermicroscopic (LM→SEM‐→TEM) correlative procedure for the examination of mammalian skeletal muscle
Author(s) -
Vriend R. A.,
Geissinger H. D.
Publication year - 1980
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1980.tb04119.x
Subject(s) - scanning electron microscope , transmission electron microscopy , ultrastructure , chemistry , materials science , osmium tetroxide , osmium , skeletal muscle , anatomy , electron microscope , biomedical engineering , biophysics , crystallography , nanotechnology , biology , composite material , optics , biochemistry , physics , medicine , ruthenium , catalysis
SUMMARY The procedure for direct intermicroscopic (LM→SEM→TEM) correlation of muscle was modified. The major modification was the use of cryostat sections which showed far better ultrastructural preservation than did paraffin sections. The use of an osmium‐thiocarbohydrazide ligand binding procedure (OTOTO) did not noticeably improve the scanning electron microscopic (SEM) image, but did result in a subsequent transmission electron microscopic (TEM) image which showed increased contrast of the intracellular membranous components. Besides improving the preservation at the TEM level of examination, the techniques used to locate the same structure or area previously examined under the complementary modes of microscopy (i.e. LM and SEM), were also refined.