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Specific techniques for the identification of O‐acylated sialic acids in colonic mucins
Author(s) -
Culling C. F. A.,
Reid P. E.
Publication year - 1980
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1980.tb04113.x
Subject(s) - mucin , biochemistry , chemistry , sialic acid , reactivity (psychology) , glycoprotein , biology , pathology , medicine , alternative medicine
SUMMARY The development of methodology for the histochemistry of mucins based upon their PAS reactivity is discussed in terms of mechanism, specificity and application. Two new histochemical methods (PB/KOH/PAS and PAT/KOH/PAS), supplemented by a variety of new and standard histochemical techniques, and correlated by parallel chemical studies, were used to demonstrate and identify C 4 and side chain O‐acylated sialic acids in colonic epithelial mucins. The application of these methods in the field of histopathology is discussed.