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Mechanisms of the Feulgen acid hydrolysis
Author(s) -
Kjellstrand Per
Publication year - 1980
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1980.tb04110.x
Subject(s) - hydrolysis , feulgen stain , chemistry , acid hydrolysis , chromatography , organic chemistry , biochemistry , dna
SUMMARY Our present knowledge of the two main reactions responsible for the form of the Feulgen hydrolysis curve is summarized. A suggestion that hydrolysis slightly above room temperature and in 5 M HCl is preferable is made and discussed. A formula making it possible to calculate the appropriate hydrolysis time at any normally used acid concentration and temperature is presented.