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Intensification of nickel‐ and cobalt‐filled neurone profiles following differential staining by rubeanic acid
Author(s) -
Quicke D. L. J.,
Brace R. C.,
Kirby P.
Publication year - 1980
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1980.tb04096.x
Subject(s) - nickel , cobalt , staining , chemistry , resolution (logic) , ammonium , nuclear chemistry , inorganic chemistry , biology , organic chemistry , computer science , genetics , artificial intelligence
SUMMARY It is demonstrated that a double colour labelling technique which uses rubeanic acid to precipitate nickel and cobalt ions within neurones (Quicke & Brace, 1979), yields complexes which may be intensified using Timm's silver method, thus allowing the subsequent appreciation of fine dendritic and axonal morphology. The resolution of neural architecture appears comparable to that revealed by the intensification of ammonium sulphide treated preparations.