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Diffusible ion localization by ion microscopy: a comparison of chemically prepared and fast‐frozen, freeze‐dried, unfixed liver sections
Author(s) -
Stika Katherine M.,
Bielat Kenneth L.,
Morrison George H.
Publication year - 1980
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1980.tb00290.x
Subject(s) - ion , chemistry , potassium , redistribution (election) , analytical chemistry (journal) , resolution (logic) , microscopy , sodium , chromatography , optics , physics , organic chemistry , artificial intelligence , politics , political science , computer science , law
SUMMARY The necessity of low temperature preparative procedures for diffusible ion localization using the ion microscope has been established with rat liver as the test material. Using conventional fixation procedures, significant ion loss and redistribution were observed which exceeded the 1 μm lateral resolution of the direct imaging ion microanalyser. The compositional morphology evidenced in chemically prepared versus fast‐frozen, freeze‐dried, unfixed material was compared and evaluated in terms of ion retention, ion redistribution and morphological integrity. A mass spectral analysis of both preparations has been presented and discussed with regard to spectral interferences and elemental sensitivities. Ion images of sodium and potassium were recorded for chemically prepared liver sections and compared with the ion distributions obtained for unfixed, fast‐frozen, freeze‐dried 1 μm thick sections. The magnitude of ion loss during preparation of samples removed at different stages in the course of chemical processing was determined by atomic absorption and flame emission spectrophotometry.