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A simple preparation, for microscopical investigations, of monolayered cells at specific cell‐cycle stages
Author(s) -
Gwynn I. Ap,
Meredith R. W. J.
Publication year - 1980
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1980.tb00261.x
Subject(s) - mitosis , usable , microbiology and biotechnology , cell , cell culture , simple (philosophy) , chemistry , materials science , biology , biophysics , computer science , biochemistry , genetics , philosophy , epistemology , world wide web
SUMMARY A technique is described whereby cells at the mitotic stage are washed off into specially designed culture tubes. Because these tubes have a small culturing area in relation to the volume of medium they contain, and the area of the original culture flask, a usable number of cell‐cycle synchronized cells are obtainable for microscopical study.