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On defining the thermal history of cells during the freezing of biological materials
Author(s) -
Bald W. B.,
Crowley A. B.
Publication year - 1979
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1979.tb04696.x
Subject(s) - thermal , finite difference scheme , ultrastructure , biological materials , sample (material) , materials science , cell , mechanics , chemistry , thermodynamics , biological system , biology , physics , anatomy , biochemistry
SUMMARY An explicit numerical finite difference scheme for defining the thermal history of a cell or group of cells contained within a biological sample of prescribed shape is described. Solutions are presented for three different cases of freezing of biological materials prior to microscopic examination. It is concluded that a surface boundary condition must be specified in any experiment before cell ultrastructure or viability can be related to thermal history or cooling rate.