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Modification of the Reichert FC‐2 cryoattachment to improve ultrathin sectioning reproducibility
Author(s) -
Leunissen J. L. M.,
Pluygers H. W.,
Elbers P. F.
Publication year - 1979
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1979.tb04692.x
Subject(s) - reproducibility , brass , materials science , biomedical engineering , composite material , metallurgy , chemistry , chromatography , copper , medicine
SUMMARY Modifications of the Reichert FC‐2 cryomicrotome attachment which facilitate sectioning of frozen material are described. Most valuable was the construction of a specimen holder chuck made of brass and connected to the specimen arm with a glass rod. Other changes include improved shielding from heat influx, a constant nitrogen pressure cooling system and a stable cryochamber mount. These modifications considerably improve reproducibility.