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Negative staining‐carbon replica method, a new procedure to reveal ultrastructure of freeze‐fractured membrane
Author(s) -
Fujikawa Seizo
Publication year - 1979
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1979.tb01182.x
Subject(s) - ultrastructure , staining , replica , negative stain , membrane , carbon fibers , dissolution , chemistry , replication (statistics) , biophysics , materials science , biology , composite material , anatomy , electron microscope , optics , biochemistry , physics , art , composite number , virology , visual arts , genetics
SUMMARY The present study was undertaken to report a new method which reveals the ultrastructure of freeze‐fractured membrane faces. The method consists of initial replication of fractured surfaces of frozen specimens with evaporated carbon and treatment of the replicas with a negative staining solution after dissolution of specimens. This negative staining‐carbon replica method reveals clear distribution, ultrastructures and precise size of intramembrane particles on the fractured membrane faces by means of density difference of negative staining solution which is deposited on the surfaces of carbon replicas.