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Spectral Changes Of The Fluorescent Acriflavine‐(Feulgen) Dna Dye Complex At Different Temperatures: Application Of A New Cryostat For Measurements In Reflected And Transmitted Light
Author(s) -
Tiffe H. W.,
Matzke K. H.,
Thiessen G.
Publication year - 1979
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1979.tb00223.x
Subject(s) - acriflavine , cryostat , fluorescence , feulgen stain , fluorescent light , biophysics , chemistry , dna , optics , materials science , biology , biochemistry , physics , superconductivity , quantum mechanics
SUMMARY The fluorometric behaviour of cellular objects is influenced during excitation by two nearly independent phenomena: (1) by the photochemical reaction of the DNA/AF dye complex, and (2) by the energy transfer among several DNA/AF dye complexes. Both processes show a distinct temperature‐dependent behaviour and can therefore be characterized by the analysis of the fluorescence spectra at different temperatures. All microfluorometric measurements were performed with a self‐constructed cooling device. The cryostat permits measurements of the cellular fluorescence within a range of temperatures between 4 K and 300 K. The cooling unit operates in accordance with the‘Continuous Flow Principle’ and allows the application of objectives up to a numerical aperture of 0·6.