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A Method For Preparing And Handling Cross‐Sections From Early Chick Embryos For Scanning Electron Microscopy
Author(s) -
Nagele R. G.,
Goldstein M. M.,
Lee H.
Publication year - 1979
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1979.tb00212.x
Subject(s) - scanning electron microscope , embryo , materials science , electron microscope , brittleness , freon , biomedical engineering , composite material , chemistry , biology , optics , microbiology and biotechnology , physics , engineering , organic chemistry
SUMMARY A method is described for processing multiple cross‐sections from early chick embryos for scanning electron microscopy. Embryos are cut through the desired regions. Sections are affixed to a coverslip with Duco cement and critical point dried by Freon 13 or liquid CO 2 . This method provides a reliable means for preparing multiple cross‐sections from single embryos and eliminates the need for direct handling of brittle tissues after drying.