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A Double Embedding Technique For The Electron Microscopic Analysis Of The Liver Acinus
Author(s) -
Miller Deborah L.,
Gumucio Jorge J.
Publication year - 1979
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1979.tb00179.x
Subject(s) - acinus , microtome , ultrastructure , electron microscope , hepatocyte , materials science , biophysics , biomedical engineering , anatomy , chemistry , pathology , biology , optics , physics , medicine , biochemistry , in vitro , pancreas
SUMMARY A double embedding technique allowing accurate and systematic sampling of zones of the hepatic acinus by electron microscopy was developed. The method involved the primary embedding of large (10 mm × 30 mm × 0·5 mm) liver slices and the preparation of 10 μm thick tissue sections by means of a rotary microtome with a steel knife. Veins 20–40 μm in diameter, the landmarks of the hepatic acinus, were localized by light microscopy, dissected from surrounding tissue and re‐embedded for ultramicrotomy. The method facilitated the systematic evaluation of hepatocyte ultrastructure in each zone of the microcirculatory unit of the liver, the hepatic acinus.