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A Scanning Microinterferometer With Correction Of Errors Caused By Overlapping ‘Ghost’ Images
Author(s) -
Jong J. E. De Josselin,
Loeve J.,
Richter H.,
Sterke H.,
Ploem J. S.
Publication year - 1979
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1979.tb00177.x
Subject(s) - optics , reproducibility , point (geometry) , microscope , standard deviation , interference (communication) , refractometer , physics , optical path length , object (grammar) , artificial intelligence , computer science , mathematics , geometry , statistics , telecommunications , channel (broadcasting) , refractive index
SUMMARY An experimental setup has been built to correct the errors caused by overlapping‘ghost’ images when scanning and integrating measurements are carried out with the Jamin‐Lebedef interference microscope. First a reference field with the object, producing the‘ghost’ image, is scanned and the values of the optical path difference (OPD) of each point in the reference field are stored in a computer. Subsequently, the OPD of the object to be measured is calculated for each point by adding the measuring result to the average OPD of five points, located around the corresponding point in the reference field. The applicability of the setup has been tested by measurements with Sepharose beads, Hela cells and the Zeiss microinterference refractometer. The difference between measurements on the same object with and without overlapping‘ghost’ image was about 10%. The reproducibility was tested by repeated measurements on the same cell whereby a standard deviation of 1·1% was found.