Premium
Lead And Uranium Stain Artefacts In Electron Microscopy: A Technique For Minimizing Their Occurrence
Author(s) -
Thaete Larry G.
Publication year - 1979
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1979.tb00170.x
Subject(s) - stain , staining , negative stain , electron microscope , uranium , microscopy , materials science , chemistry , optics , mineralogy , computer science , pathology , physics , metallurgy , medicine
SUMMARY A new technique is presented which significantly reduced the occurrence of artefacts by eliminating the air‐stain interface when staining thin sections of biological material. It is designed for simultaneous treatment of large numbers of samples. The experiment described deals mainly with the specific staining problems encountered when using grids coated with Formvar.