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A New Method For Scanning Electron Microscopic Visualization Of Dermal Elastic Fibres
Author(s) -
Tsuji Takuo,
Lavker Robert M.,
Kligman Albert M.
Publication year - 1979
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1979.tb00166.x
Subject(s) - dermis , scanning electron microscope , ultrastructure , materials science , fixation (population genetics) , electron microscope , composite material , chemistry , anatomy , optics , biology , physics , biochemistry , gene
SUMMARY Autoclaving sections of dermis for 8 h followed by fixation, dehydration and xylol‐air drying yields a pure preparation of elastic fibres for scanning electron microscopy which retains the native architecture of this component. Elastic fibres were intertwined in a complex fashion with numerous branches. Fibres were predominantly cylindrical in the upper dermis and became larger and more elliptical in the deeper dermis. This method produces a means to study the organization of elastic fibres in a variety of disorders in which dermal changes are prominent.