Premium
A Rapid Method For Estimating Volume Ratios
Author(s) -
Orive L. M. Cruz,
Myking A. O.
Publication year - 1979
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1979.tb00162.x
Subject(s) - mathematics , stereology , volume (thermodynamics) , robustness (evolution) , statistics , star (game theory) , population , standard deviation , constant (computer programming) , analytical chemistry (journal) , relative standard deviation , combinatorics , materials science , physics , chemistry , mathematical analysis , computer science , chromatography , biology , thermodynamics , biochemistry , demography , sociology , detection limit , gene , programming language , endocrinology
SUMMARY A rapid stereological method is proposed for estimating the volume ratio V ( K 2 )/ V (K 1 ) of two bodies K 1 , K 2 such that K 2 is embedded in K 1 . If K 1 , K 2 fit approximately into a ‘star specimen’ model, then a single section, taken at a certain level through K 1 , provides a rather accurate estimate of V (K 2 )/ V (K 1 ). For a population of moderately similar star specimens, a constant sectioning level can be estimated from a sample using a least squares criterion. A pilot experiment involving mouse lymph nodes, aimed at estimating the mean and the variance of the individual fractions V (paracortex)/ V (node), indicates a fair robustness against deviations from the model.