Premium
Multiple marking of cell surface receptors by gold granules: simultaneous localization of three lectin receptors on human erythrocytes
Author(s) -
Horisberger Marc,
Vonlanthen Monique
Publication year - 1979
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1979.tb00155.x
Subject(s) - lectin , concanavalin a , receptor , wheat germ agglutinin , biophysics , cell , colloidal gold , cell surface receptor , resolution (logic) , transmission electron microscopy , chemistry , biology , biochemistry , materials science , nanotechnology , in vitro , nanoparticle , artificial intelligence , computer science
SUMMARY A method is described for transmission electron microscopy which allows high resolution mapping of three lectin receptors on the same cell. As a model, receptors for Concanavalin A, soya bean and wheat germ agglutinins have been simultaneously localized on human erythrocytes using lectin‐labelled gold markers of different sizes (5, 17 and 26 nm, respectively). Quantitative binding data and examination of stereoscopic micrographs of thin sections of marked erythrocytes indicated that the greatest part of these lectin markers was bound by receptors spatially separated. As gold granules are electron dense and uniform in size, they are well suited for multiple marking of cell surface components. The method is simple and leads to a better understanding of the topography of cell membranes.