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A simple freeze‐drying technique for preparing biological tissue without chemical fixation for electron microscopy
Author(s) -
Edelmann Ludwig
Publication year - 1978
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1978.tb01172.x
Subject(s) - biological tissue , simple (philosophy) , fixation (population genetics) , electron microscope , materials science , biological specimen , biomedical engineering , nanotechnology , chemistry , optics , physics , biochemistry , medicine , philosophy , epistemology , gene
SUMMARY A simple technique for freeze‐drying and embedding of small pieces of biological tissue at low temperature is described, utilizing exclusively cryosorption pumping. Good preservation of chemically unfixed tissue is observed.