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A simple and inexpensive culture device for cell growth, quantitative microscopy and autoradiography
Author(s) -
Fox Cecil H.,
Auer Gert,
Zetterberg A.,
CottlerFox M.
Publication year - 1978
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1978.tb01171.x
Subject(s) - gasket , hypodermic needle , materials science , microscopy , biomedical engineering , silicone rubber , composite material , pathology , medicine , syringe , psychiatry
SUMMARY An inexpensive culture chamber suitable for subsequent preparations for cyto‐chemical or radiographic work is demonstrated. The chamber consists of two glass microscope slides suitably prepared for cell growth and a gasket of silicone rubber. The slides and gasket are sealed and held together with two ordinary spring letter clips or clothes‐pins. Cells may be inoculated by piercing the gasket with a hypodermic needle, or by removing the top slide and adding cells directly. Long‐term cultivation with medium replacement is easy and efficient and addition of isotopes or other materials may be done aseptically.