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Quantitative X‐ray microanalysis of diffusible ions in the skeletal muscle bulk specimen
Author(s) -
Zierold K.,
Schäfer D.
Publication year - 1978
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1978.tb01156.x
Subject(s) - microanalysis , fixative , electrolyte , ion , electron probe microanalysis , analytical chemistry (journal) , chemistry , electron microscope , x ray , scanning electron microscope , materials science , electron microprobe , chromatography , mineralogy , biochemistry , optics , physics , organic chemistry , electrode , cytoplasm , composite material
SUMMARY X‐ray microanalysis in the scanning electron microscope (SEM) is used to obtain information about the distribution and mobility of electrolyte ions in the skeletal muscle tissue. In order to localize the diffusible ions in their physiological state, so far as possible, any chemical fixative is avoided. A special ‘cooling chain’ preparation method is applied which enables studies on cryofractured bulk specimens at low temperatures. Quantitation of the obtained X‐ray spectra is done by the use of glycerol‐treated tissue specimens which are incubated with electrolyte solutions of defined ion concentration.