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Instrumentation and specimen preparation for electron beam X‐ray microanalysis of frozen hydrated bulk specimens
Author(s) -
Fuchs Wolfgang,
Lindemann Bernd,
Brombach Jobst D.,
Trösch Walter
Publication year - 1978
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1978.tb01155.x
Subject(s) - microanalysis , materials science , liquid nitrogen , analyser , instrumentation (computer programming) , cathode ray , scanning electron microscope , biological specimen , electron microscope , analytical chemistry (journal) , composite material , electron , chemistry , optics , chromatography , physics , organic chemistry , quantum mechanics , computer science , operating system
SUMMARY A cooling chain for the handling of frozen hydrated bulk specimens is described. Use of this method permits the specimen to be kept fully hydrated. After quench freezing, the specimen is transferred to a freeze etch apparatus, freeze fractured, carbon coated and transferred onto the precooled cold stage of the SEM by means of an airlock. The specimen is examined in the secondary electron mode and analysed using an energy dispersive X‐ray analyser. The midgut of Chironomus thummi larvae and frog skin epithelium were used to test the performance.