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Ponceau 2R staining of proteins and periodic acid bleaching of osmicated subcellular structures on semi‐thin sections of tissues processed for electron microscopy: a simplified procedure
Author(s) -
Gori Paolo
Publication year - 1978
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1978.tb00122.x
Subject(s) - osmium tetroxide , staining , glutaraldehyde , bleach , stain , periodic acid , chemistry , negative stain , electron microscope , osmium , alizarin red , periodic acid–schiff stain , aqueous solution , chromatography , biochemistry , optics , organic chemistry , biology , catalysis , physics , genetics , ruthenium
SUMMARY An aqueous solution (pH 1·5) of 0·5% Ponceau 2R (C.I. 16150) and 2% periodic acid was used at 318 K to stain proteins (brilliant red) and simultaneously to bleach the osmicated non‐proteinaceous cell structures on 1–2 μm thick sections of tissues fixed in glutaraldehyde‐osmium tetroxide and embedded in epoxy resins. This H 5 IO 6 bleaching‐Ponceau 2R staining procedure only stains the proteins so that black‐and‐white films can be used.