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A simple method for maintaining relative positions of separate tissue elements during processing for electron microscopy
Author(s) -
Stirling Charles A.
Publication year - 1978
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1978.tb00121.x
Subject(s) - electron microscope , osmium , microscopy , materials science , osmium tetroxide , epoxy , fixation (population genetics) , chemistry , composite material , optics , physics , biochemistry , ruthenium , gene , catalysis
SUMMARY Molten (328 K) 20% gelatin is used as a ‘glue’ to hold together separate tissue elements or tissue elements that may be separated when cutting small blocks of tissue for plastic embedding. Standard aldehyde and osmium fixation, dehydration and epoxy embedding are compatible with this as is semi‐thin sectioning for light microscopy or thin sectioning for electron microscopy.