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Extraction of glycoproteins during tissue preparation for electron microscopy
Author(s) -
Sturgess J. M.,
Mitranic M. M.,
Moscarello M. A.
Publication year - 1978
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1978.tb00120.x
Subject(s) - electron microscope , glycoprotein , extraction (chemistry) , chemistry , microscopy , materials science , nanotechnology , biophysics , chromatography , biology , physics , biochemistry , optics
SUMMARY The extraction of glycoproteins labelled with 3 H or 14 C precursors: fucose and glucosamine, has been compared during processing of rat liver for transmission electron microscopy. Using 14 C labelled fucose, approximately 6% of labelled macromolecules were extracted during processing including 3% during glutaraldehyde fixation, 1% during post‐fixation and 2% during dehydration. Greater extraction (8%) occurs with glucosamine as a precursor, which may be attributed to hydrolysis of the more labile sialic acid residues of the glycoprotein molecules. Tritium labelled glycoproteins were more susceptible to extraction than 14 C‐labelled glycoproteins. The extraction of 3 H‐labelled glycoproteins as measured by liquid scintillation counting may prove difficult to interpret owing to the quenching from processing solutions.