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The use of PIPES buffer in the fixation of mammalian and marine tissues for electron microscopy
Author(s) -
Baur P. S.,
Stacey T. R.
Publication year - 1977
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1977.tb01145.x
Subject(s) - buffer (optical fiber) , ultrastructure , electron microscope , fixation (population genetics) , materials science , chemistry , biophysics , biology , optics , biochemistry , computer science , anatomy , physics , telecommunications , gene
SUMMARY PIPES, an organic based buffer, was used in the preparation of a variety of marine and mammalian tissues for electron microscopic study. The ultrastructural results demonstrate that this buffer renders superior ultrastructural details to those obtained with some of the more common inorganic buffers especially when long fixation times are required. Furthermore, this buffer system does not appear to contribute extraneous anions or cations to the tissues and thus permits accurate elemental determinations to be performed on thin sections of the plastic embedded specimens by means of energy dispersive X‐ray analysis.