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Staining reticulin in beef dorsal lymph nodes for electron microscopy
Author(s) -
Voyle C. A.
Publication year - 1977
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1977.tb01135.x
Subject(s) - electron microscope , pathology , staining , dorsum , anatomy , microscopy , reticular fiber , h&e stain , negative stain , chemistry , lymph , biology , medicine , optics , physics
SUMMARY Reticulin has been classically identified by virtue of its argyrophilia, as observed by light microscopy. The similarity between reticulin and collagen has been a matter for considerable discussion. This paper describes the application of a silver‐staining procedure which demonstrates the fine structure of these fibres when studied by electron microscopy, giving a direct indication of the morphological identity of the argyrophilic structures, i.e. reticulin, with collagen. Beef lymph node tissue was used for this study.