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Detection of Concanavalin A receptors by affinity to peroxidase and iron dextran by scanning and transmission electron microscopy and X‐ray microanalysis
Author(s) -
Baccetti Baccio,
Burrini Anna G.
Publication year - 1977
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1977.tb01130.x
Subject(s) - concanavalin a , microanalysis , transmission electron microscopy , peroxidase , scanning electron microscope , chemistry , electron microscope , analytical chemistry (journal) , biophysics , crystallography , materials science , optics , nanotechnology , chromatography , biochemistry , enzyme , biology , physics , organic chemistry , in vitro
SUMMARY A method of cell surface mapping has been developed on spermatozoa. Concanavalin A binding sites have been simultaneously revealed both by peroxidase DAB reaction and by iron dextran coupling. The areas are examined in scanning and transmission electron microscopes and submitted to electron probe X‐ray microanalysis.