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Freeze‐drying process of biological specimens observed with a scanning electron microscope
Author(s) -
Nei Tokio,
Fujikawa Seizo
Publication year - 1977
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1977.tb00054.x
Subject(s) - shrinkage , freeze drying , scanning electron microscope , dehydration , parenchyma , electron microscope , process (computing) , environmental scanning electron microscope , membrane , materials science , chemistry , biophysics , composite material , biology , botany , chromatography , optics , biochemistry , physics , computer science , operating system
SUMMARY A pit membrane was observed with a cryo‐SEM during the course of dehydration at low temperatures. The freeze‐drying process of sea‐urchin eggs and parenchyma cells of higher plants was also examined with this microscope. Conditions for observation of frozen specimens in the native state were discussed on the basis of morphological studies of alterations such as shrinkage or deformation which appeared during the freeze‐drying process.