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Ponceau 2R staining on semi‐thin sections of tissues fixed in glutaraldehyde‐osmium tetroxide and embedded in epoxy resins
Author(s) -
Gori Paolo
Publication year - 1977
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1977.tb00028.x
Subject(s) - osmium tetroxide , glutaraldehyde , staining , epoxy , osmium , chemistry , nuclear chemistry , materials science , polymer chemistry , chromatography , organic chemistry , catalysis , electron microscope , pathology , optics , medicine , ruthenium , physics
SUMMARY Proteins were stained (brilliant red) by using 0·5% Ponceau 2R (C.I. 16150) in sulphuric acid (pH 2) at 40°C on sections 1–2 μm thick of tissues fixed in glutaraldehyde‐osmium tetroxide and embedded in epoxy resins. The staining is free from precipitates and does not require the removal of the embedding media. Section bleaching with 1–2% periodic acid at 40°C for 30–90 min was also carried out in order to obtain a more brilliant colour of the proteins which may be photographed using black and white films.