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X‐ray microanalysis of ultrathin frozen and freeze‐dried sections of human sperm cells
Author(s) -
Chandler J. A.,
Battersby S.
Publication year - 1976
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1976.tb02423.x
Subject(s) - microanalysis , sperm , microchemistry , chemistry , materials science , biology , chromatography , botany , organic chemistry
SUMMARY X‐ray microanalysis of air dried and ultrathin frozen and frozen dried sections of human sperm cells has indicated a large deviation of elemental composition between cells in a single sample and between samples. Analysis of air dried cells indicates a similar subcellular elemental distribution to that found in sectioned material. Sodium to potassium ratios are found to be similar in both preparations. Air drying is considered a valid method for the preparation of sperm cells for analysis.