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Cell surgery by laser micro‐dissection: A preparative method
Author(s) -
Isenberg G.,
Bielser W.,
MeierRuge W.,
Remy E.
Publication year - 1976
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1976.tb02419.x
Subject(s) - laser , cytoplasm , organelle , dissection (medical) , cell , fibril , irradiation , laser beams , materials science , biomedical engineering , optics , chemistry , biophysics , anatomy , biology , physics , medicine , biochemistry , nuclear physics
SUMMARY A new UV‐laser device is introduced as a micro‐manipulator and micro‐dissection apparatus for experimental cell research. High energy irradiation is produced by a quasi‐continuous N 2 ‐laser. The laser micro‐beam can be focused to a minimal diameter of 0.75 μm. The micro‐laser can be used for cutting and, hence, as a micro‐surgical instrument at the level of cellular organelles. Cytoplasmic actomyosin fibrils (AM fibrils) from tissue culture cells (RMCD–cells) are isolated from the surrounding ground cytoplasm. It is shown that the contractile proteins are not damaged by the laser dissection method to an extent that would prevent their normal function, e.g. contraction.