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High‐resolution autoradiography of 3 H‐ouabain binding in salt transporting epithelia
Author(s) -
Stirling C. E.
Publication year - 1976
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1976.tb02397.x
Subject(s) - ouabain , killifish , gill , chemistry , enterocyte , resolution (logic) , biophysics , mucus , biology , biochemistry , sodium , fish <actinopterygii> , small intestine , ecology , organic chemistry , artificial intelligence , fishery , computer science
SUMMARY The distribution of salt transporting sites in epithelia is a problem of special interest to electrolyte metabolism. Autoradiography of freeze‐dried plastic embedded tissue was used to localize 3 H‐ouabain in rabbit small intestine and renal medulla and gills of killifish. Resolution approaching theoretical limits for tritium at the light microscope level was achieved. The results of several tests are presented which demonstrate the resolution of the method and the specificity of the observed ouabain binding patterns. A large uptake of ouabain was found along the basolateral membranes of the enterocyte, thick ascending tubules and chloride cells (fish gill).