Premium
EM autoradiography of non‐sectioned biological material
Author(s) -
Haworth R. A.,
Chapman J. A.
Publication year - 1976
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1976.tb02395.x
Subject(s) - emulsion , materials science , suspension (topology) , biological materials , resolution (logic) , biological specimen , collagen fibril , high resolution , biomedical engineering , chemistry , composite material , optics , biophysics , computer science , biology , physics , mathematics , geology , biochemistry , remote sensing , artificial intelligence , homotopy , pure mathematics , medicine
SUMMARY Methods of EM autoradiography suitable for the study of non‐sectioned biological material deposited on a support film from suspension are described. The methods, which include modified procedures for specimen support and emulsion preparation, may also have advantages for the study of any specimen where physical separation of specimen and emulsion is desirable. In a study of the growth of 125 I‐labelled collagen fibrils reconstituted from solution in vitro , these procedures have allowed the production of EM autoradiographs which show a high resolution and are free from stress artefacts and chemography.