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A method of embedding tissue culture preparations in situ for transmission electron microscopy
Author(s) -
Codling B. W.,
Mitchell J. C.
Publication year - 1976
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1976.tb02390.x
Subject(s) - araldite , transmission electron microscopy , electron microscope , materials science , in situ , microscopy , embedding , filter (signal processing) , optics , chemistry , physics , nanotechnology , computer science , artificial intelligence , adhesive , organic chemistry , layer (electronics) , computer vision
SUMMARY A method for embedding tissue culture preparations, grown on Millipore filters, in Araldite is described. Accurate light microscopic localization of areas suitable for ultrathin sectioning is possible. The plane of sectioning relative to the Millipore filter can be varied to suit individual experimental needs.