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Correction of stereological parameters from biased samples on nucleated particle phases
Author(s) -
Orive LuisM. Cruz
Publication year - 1976
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1976.tb02379.x
Subject(s) - surface (topology) , nucleus , particle (ecology) , yield (engineering) , volume (thermodynamics) , simple (philosophy) , product (mathematics) , computational physics , materials science , mathematics , physics , statistical physics , chemistry , geometry , thermodynamics , geology , philosophy , oceanography , epistemology , biology , microbiology and biotechnology
SUMMARY General formulations for correcting component‐biased S/V estimates on poly‐dispersed phases of nucleated particles are set out. Direct application of these corrections to monodispersed phases where nucleus and cell are spheroids, yield explicit corrections. As a rule, the corrected specific surface area of the containing bodies equals the product of the nuclear‐biased estimate times a correction coefficient, which is bigger than one, and depends upon the shape of the components and the relative volume of nucleus‐in‐cell. Reference curves which allow a rapid obtention of the correction factor, are provided. Simple formulae for correcting biased surface density and surface‐to‐volume ratio estimates other than the specific surface area, are also given, together with the standard errors of the corrected parameters.