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An improved method for photographing fluorescent human chromosomes *
Author(s) -
Verma Ram S.,
Dosik Harvey
Publication year - 1976
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1976.tb01107.x
Subject(s) - photography , fluorescence , filter (signal processing) , computer science , optics , light source , materials science , computer vision , physics , art , visual arts
SUMMARY In the photography of sequentially QFQ and RFA banded chromosomes, the fading problem can be solved by reducing exposure time. This can be accomplished by choosing the right light source, filter combinations, type of film and developer. Three different systems were employed to record the exposure time during photography. A ‘Vertical Illuminator III RS’ with a 50 w/AC mercury burner equipped with 400–490, BG 12–BG 38 excitation filters and 510 barrier filter was found to be the most efficient and satisfactory. QFQ banded chromosomes can be photographed within 3–5 s and RFA banded chromosomes within 10–15 s with this system.