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A technique for electron microscope autoradiography (and X‐ray microanalysis) of diffusible substances using freeze‐dried fresh frozen sections
Author(s) -
Baker J. R. J.,
Appleton T. C.
Publication year - 1976
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1976.tb01102.x
Subject(s) - microanalysis , electron microscope , electron probe microanalysis , biological specimen , ultrastructure , microscopy , chemistry , microscope , scanning electron microscope , radiochemistry , biophysics , materials science , mineralogy , biology , optics , electron microprobe , anatomy , physics , organic chemistry , composite material
SUMMARY Several attempts have been made over the past few years to localize diffusible radioactive substances in tissues at the electron microscope level. The procedures employed hitherto have involved one or more aspects of the methods used in tissue preparation for conventional electron microscopy and as such are unlikely to be universally applicable for all classes of diffusible substance. The present paper shows that the use of ultrathin frozen sections of fresh unembedded biological material permits the ultrastructural localization by autoradiography and X‐ray microanalysis of highly soluble electrolytes.