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Wholebody autoradiography: a β‐radioluminescence method for detecting tritium
Author(s) -
Farebrother D. A.,
Creasy Dianne M.
Publication year - 1976
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1976.tb01090.x
Subject(s) - radioluminescence , tritium , scintillator , radiochemistry , beta particle , materials science , phosphor , nuclear medicine , chemistry , physics , optics , nuclear physics , medicine , detector , optoelectronics
SUMMARY A method of increasing the sensitivity of X‐ray film for the wholebody autoradiography of tritiated compounds is described. Thirty micrometre sections are sprayed with a scintillator, Diphenyl oxazole (PPO) and exposed to light sensitive (screen) X‐ray film at −70°C. The method permits a substantial reduction in the required dose of tritium and of the period of exposure of tissue sections.