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Scanning electron microscopy of renal vascular casts
Author(s) -
Bielke S. R.,
Nagle R. B.,
Trump B. F.,
Bulger R. E.
Publication year - 1976
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1976.tb01082.x
Subject(s) - scanning electron microscope , perfusion , kidney , fixation (population genetics) , pathology , chemistry , biomedical engineering , materials science , anatomy , medicine , composite material , biochemistry , gene
SUMMARY The use of Cementex to form casts of the renal vascular system is described. These casts are useful in delineating the vascular microarchitecture of the kidney including that of the glomerular capillaries, but do not appear to reproducibly reflect physiological vascular tone. The postglomerular vascular system is often incompletely filled using this technique. The amount of filling can be altered by the techniques used in cast preparation. The effect of perfusion pressure, concentration of Cementex, fixation, and type of colour additive are compared with respect to the cast structure.