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Handling and staining epoxy resin sections for light microscopy
Author(s) -
Roberts I. M.,
Hutcheson A. M.
Publication year - 1975
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1975.tb04543.x
Subject(s) - epoxy , materials science , stain , electron microscope , microscopy , composite material , staining , scanning electron microscope , optical microscope , optics , pathology , medicine , physics
SUMMARY Epoxy resin sections 0.1‐1.0 μm thick of specimens embedded for electron microscopy, were collected from the ultramicrotome using strips of cover slip, and, after drying on a hot plate, were bulk stained in specially made troughs. After treatment with periodic acid, the sections were stained first in basic fuchsin at 70°C and then in alkaline methylene blue at room temperature. The handling technique allows accurate collection of serial sections without interrupting the sectioning process, and the stain combination is suitable for different specimens in different epoxy resins and is unaffected by storage for more than 1 year.