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Preparation of nematodes for scanning electron microscopy
Author(s) -
Green C. D.,
Stone A. R.,
Turner R. H.,
Clark S. A.
Publication year - 1975
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1975.tb04540.x
Subject(s) - scanning electron microscope , epoxy , tylenchida , materials science , acetone , electron microscope , rhabditida , chemistry , composite material , biology , botany , nematode , optics , biological pest control , nematology , organic chemistry , physics , ecology
SUMMARY Nematodes from the orders Tylenchida and Rhabditida were fixed and processed in several different ways for examination with the scanning electron microscope (SEM). Four processes produced good preparations of fixed nematodes. Drying from acetone was the simplest of these techniques and most useful for regions of the tylenchid nematodes supported by skeletal tissue. Critical point drying, a more complicated procedure, gave good preparations but they required special care in processing. Nematodes infiltrated with glycerol and a conducting agent were the most life‐like but were difficult to examine. Specimens infiltrated with an epoxy resin looked natural and this was the most promising process tried.