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Identification of nerve fibres in the scanning electron microscope
Author(s) -
Sætersdal Thorvald Selmer,
Myklebust Reidar
Publication year - 1975
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1975.tb04537.x
Subject(s) - scanning electron microscope , magnification , transmission electron microscopy , electron microscope , materials science , microscopy , stain , silver stain , staining , pathology , optics , nanotechnology , composite material , medicine , physics
SUMMARY The use of silver as a ‘stain’ for nerve fibres in scanning electron microscopy (SEM) preparations has been investigated. Samples of cardiac tissues were treated according to the Bielschovsky silver impregnation method. Following embedding in paraffin, successive sections were selected for light microscopy and for SEM studies, respectively. The silver impregnated fibres, when examined in SEM preparations, appeared nearly white against a greyish background of cardiac tissue. They were therefore easy to localize even at a low magnification. These nerve fibres were identified in the same tissue, but different blocks by means of transmission electron microscopy (TEM) studies and by fluoroescence microscopy.