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Redundancy of washing in the preparation of biological specimens for transmission electron microscopy
Author(s) -
Ockleford C. D.
Publication year - 1975
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1975.tb04050.x
Subject(s) - transmission electron microscopy , trophoblast , electron microscope , biological specimen , dehydration , microscopy , ultrastructure , materials science , microbiology and biotechnology , biology , chemistry , biophysics , nanotechnology , biochemistry , anatomy , pathology , optics , placenta , medicine , physics , ecology , pregnancy , fetus , genetics
SUMMARY A marine unicellular organism, human trophoblast tissue and cultured trophoblast cells of human origin have been satisfactorily preserved for electron microscopy without resort to washing either before dehydration or between different stages of fixation. The time required to fix and dehydrate a specimen using this method is 55 min.