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Coating sections for electron microscopic autoradiography: a stripping technique using liquid emulsion
Author(s) -
Burry Richard W.,
Lasher Robert S.
Publication year - 1975
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1975.tb04029.x
Subject(s) - emulsion , electron microscope , coating , materials science , resolution (logic) , microscope , stripping (fiber) , scanning electron microscope , layer (electronics) , optics , optical microscope , photographic emulsion , chemistry , composite material , physics , biochemistry , artificial intelligence , computer science , silver halide
SUMMARY A coating technique for electron microscope autoradiography is described which combines the advantage of forming an emulsion film by a dipping method with the ease of coating sections already on grids. Sections are coated so that a formvar support film separates the section and the emulsion crystals. This intermediate layer of formvar ensures a random distribution of the emulsion crystals. Using light gold sections, Ilford L‐4 emulsion and Microdol‐X development, the resolution of this technique, as determined by the half distance method, was 150 nm. The additional layer of formvar slightly reduced the image quality with biological samples in the electron microscope. This technique has a minimal loss of resolution and image quality for moderate resolution electron microscope autoradiography.