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New techniques for the preparation of uniform layers of nuclear emulsions for use in micro‐autoradiography
Author(s) -
Sanderson J.
Publication year - 1975
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1975.tb04015.x
Subject(s) - emulsion , reproducibility , materials science , microscope , photographic emulsion , dilution , chromatography , photometer , layer (electronics) , optics , chemistry , nanotechnology , physics , silver halide , biochemistry , thermodynamics
SUMMARY A procedure is described whereby liquid emulsion can be used to coat glass coverslips to produce films of emulsion suitable for quantitative evaluation of grain density in micro‐autoradiographs. Any thickness of emulsion layer can be produced from 3 μm upwards, depending on the volume and dilution of the emulsion dispensed. The uniformity and reproducibility of the films have been tested by observing light transmission of undeveloped preparations using a microscope photometer.