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A technique for preparing human dermal and scar specimens for scanning electron microscopy
Author(s) -
Sommerlad B. C.,
Creasey J. M.
Publication year - 1975
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1975.tb03992.x
Subject(s) - scanning electron microscope , materials science , glutaraldehyde , fixation (population genetics) , biomedical engineering , dermis , microscopy , electron microscope , composite material , optics , anatomy , chemistry , chromatography , medicine , biochemistry , physics , gene
SUMMARY The aims in a current study of dermis and scar tissue using the scanning electron microscope are:1 to maintain the orientation of the specimen 2 to preserve the in vivo architecture, and 3 to expose a satisfactory surface.A technique which best fulfils these requirements involves glutaraldehyde fixation of a splinted specimen, freeze‐fracturing using a modified pair of pliers, freeze‐drying, incubation in a buffered saline solution of hyaluronidase and freeze‐drying again after washing. Consistently satisfactory specimens have been obtained, which provide information about fibre architecture and cell distribution.