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The effect of intestinal distension and of section thickness on counts of epithelial nuclei in the rat small intestine
Author(s) -
Clarke Rufus M.
Publication year - 1975
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1975.tb03989.x
Subject(s) - distension , anatomy , small intestine , epithelium , biology , hydrostatic pressure , chemistry , pathology , medicine , endocrinology , physics , thermodynamics
SUMMARY1 A method is described for counting nuclei in wax‐embedded histological sections of epithelium. The counts are independent of section thickness over a wide range (4–10 μm). 2 Counts of nuclei were made in villi and crypts of rat small intestine fixed either collapsed or distended by a hydrostatic pressure equivalent to 350 mm of water. There was no significant difference between the counts of nuclei in collapsed or distended gut. 3 Distension reduced the absolute height of villi, and increased their absolute width at the base. 4 The implications of these findings for studies of the morphological basis of intestinal adaptation are discussed.