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An autoradiographic study of the binding of 3 H‐chlorambucil to cells of a drug‐sensitive and drug‐resistant strain of the Yoshida ascites sarcoma
Author(s) -
Birbeck M. S. C.,
Robertson D.,
Hill B. T.
Publication year - 1975
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1975.tb03899.x
Subject(s) - chlorambucil , drug , ascites , chemistry , strain (injury) , drug resistance , cancer research , pharmacology , medicine , chemotherapy , biology , microbiology and biotechnology , cyclophosphamide
SUMMARY The binding of chlorambucil to drug‐sensitive and drug‐resistant Yoshida sarcoma cells has been investigated by an autoradiographic technique. The cells were treated with the drug at various concentrations both in vitro and in vivo. Using both light and electron microscope techniques, and by counting grains on various areas of the cell, the following conclusions were reached. (1) At all drug concentrations there was general cytoplasmic and nuclear uptake by both cell lines. There was no evidence of a specific localization in one site only or for specific exclusion of any one site. (2) At high dose levels (∼2 mM) the binding was approximately equal in both cell lines, although a few damaged cells bound much greater amounts of drug. (3) At low dose levels (∼3 μM) there was a higher uptake by the nucleus of the sensitive cells compared with the nucleus of the resistant cells; the cytoplasmic binding was similar.