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Contrast enhancement in biological electron microscopy using two micrographs
Author(s) -
Misell D. L.,
Burge R. E.
Publication year - 1975
Publication title -
journal of microscopy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.569
H-Index - 111
eISSN - 1365-2818
pISSN - 0022-2720
DOI - 10.1111/j.1365-2818.1975.tb03894.x
Subject(s) - electron micrographs , micrograph , electron microscope , dark field microscopy , optics , scattering , microscopy , image contrast , contrast (vision) , materials science , biological specimen , scanning electron microscope , physics
SUMMARY A method is given for either dark‐ or bright‐field electron microscopy of thin biological specimens (e.g. thin sections) for enhancing the image arising from elastic scattering, relative to the composite image formed by inelastic scattering. The method requires two micrographs of a given field taken at two particular conditions of image defocus.